Using stool samples from Viking latrines, researchers at the University of Copenhagen have genetically mapped one of the oldest human parasites – the whipworm. The mapping reflects the parasite's global spread and its interaction with human beings, a delicate relationship that can make us healthier and ill.

Using fossilized eggs in up to 2500-year-old feces from Viking settlements in Denmark and other countries, researchers at the University of Copenhagen's Department of Plant and Environmental Sciences and the Wellcome Sanger Institute (UK) have made the largest and most in-depth genetic analysis of one of the oldest parasites found in humans – the whipworm.

The study, published in Nature Communications, presents completely new knowledge about the parasite's development and prehistoric dispersal. This knowledge can be applied in efforts to prevent the parasite's drug resistance and its future spread.

The study suggests that human and parasite have developed a delicate interaction over thousands of years, whereby the parasite tries to stay "under the radar" not to be repelled, which allows it more time to infect new people. From other studies, it is known that the whipworm stimulates the human immune system and the gut microbiome, to the mutual benefit of both host and parasite.

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While whipworm (Trichuris trichiura) is now rare in industrialized countries, and most often only causes minor problems among healthy individuals, the parasite is estimated to affect 500 million people in developing countries.

In people who are malnourished or have impaired immune systems, whipworm can lead to serious illness. Our mapping of the whipworm and its genetic development makes it easier to design more effective anti-worm drugs that can be used to prevent the spread of this parasite in the world's poorest regions."

Professor Christian Kapel, UCPH's Department of Plant and Environmental Sciences

Fossilized latrine poop from Copenhagen and Viborg

Eggs, not worms, made it possible for researchers to examine the genetic material of thousands-of-years-old whipworms. Due to extremely durable chitin in egg capsules, their internal DNA has been well preserved while the eggs have been buried in moist soil.

By examining fossilized stool samples which were previously discovered in the latrines of Viking settlements in Viborg and Copenhagen, the researchers isolated the eggs under a microscope, sieved them from the stool and subjected them to refined genetic analyses that the researchers have been perfecting for years in previous studies.

"We have known for a long time that we could detect parasite eggs up to 9000 years old under a microscope. Lucky for us, the eggs are designed to survive in soil for long periods of time. Under optimal conditions, even the parasite's genetic material can be preserved extremely well. And some of the oldest eggs that we've extracted some DNA from are 5000 years old. It has been quite surprising to fully map the genome of 1000-year-old well-preserved whipworm eggs in this new study," explains Christian Kapel.

The researchers examined archaeological stool samples from several locations. These ancient genetic samples are compared with contemporary samples obtained from people with whipworms from around the world. Doing so has provided researchers with an overview of the worm's genome and its evolution over ten-thousands of years.

"Unsurprisingly, we can see that the whipworm appears to have spread from Africa to the rest of the world along with humans about 55,000 years ago, following the so-called 'out of Africa' hypothesis on human migration," explains Christian Kapel.

Can live unnoticed in the intestine for months

A whipworm can grow five to seven centimeters in length and live unnoticed in the intestine of a healthy individual for several months. During this time, it lays eggs continuously, which are expelled through feces. In people with weakened immune systems, whipworm can cause a wide range of gastrointestinal diseases, malnutrition and even delay childhood development.

Worms are transmitted via the fecal-oral route, meaning that microscopic parasite eggs in soil can spread to drinking water or food, after which they are ingested through the mouth of a new host.

"The eggs lie in the ground and develop for roughly three months. Once matured, eggs can survive in the wild for even longer, as they wait to be consumed by a new host in whose digestive tract they will then hatch. Their entire life cycle is adapted to survive in soil for as long as possible," explains Christian Kapel.

As such, the golden years for these worms in our part of the world were when our toilet and kitchen conditions, as well as personal hygiene, were significantly different than today.

"During the Viking Age and well into the Middle Ages, one didn't have very sanitary conditions or well-separated cooking and toilet facilities. This allowed the whipworm far better opportunities to spread. Today, it is very rare in the industrialized part of world. Unfortunately, favorable conditions for spreading still exist in less developed regions of the world," says Christian Kapel.

Facts:

• The new study provides the first in-depth and complete genetic mapping of the whipworm. Until now, only limited segments of the genome were known.
• The parasite spread from Africa to the rest of the world along with our human ancestors.
• Among other things, the researchers collected stool samples with whipworm eggs from Viking settlements in Denmark, Latvia and the Netherlands.
• The ancient genetic samples were compared with contemporary samples from people with whipworms from many various countries in Africa, Central America, Asia, and Europe.
• The US Center for Disease Control (CDC) estimates that 604-795 million people are infected by trichuriasis worldwide. Source: Pullan, R. L., Smith, J. L., Jasrasaria, R. & Brooker, S. J. (2014) Global numbers of infection and disease burden of soil transmitted helminth infections in 2010. Parasite. Vectors 7, 37.
• The study is led by the University of Copenhagen's Department of Plant and Environmental Sciences, Section for Organism Biology and made in collaboration with the Wellcome Sanger Institute (UK).